FIG 2.

Antigen presentation of NMI by MHC-I is partially dependent on TAP1. Deficient and WT mice were infected i.p. with 1 × 10⁷ NMI organisms. Disease severity was assessed at 14 and 28 dpi. (A) Relative body weight was determined throughout infection for monitoring disease severity. (B) Splenomegaly was determined as a ratio of spleen weight to body weight. (C) Bacterial burden in the spleen was determined by real-time PCR and reported as log₁₀ C. burnetii com1 gene copy numbers. These data indicate that TAP1-deficient mice had more severe disease following infection with NMI at 14 dpi. The data presented for each group are the average with standard deviation for four mice. Results were analyzed by t test (B and C) or two-way ANOVA with Sidak's multiple-comparison test (A) for statistical significance. *, P < 0.05; **, P < 0.01.