Fig. 3.

The Western blots in IEC-6 cells. (A) COX-2 and IL-1β levels in total cell lysate was determined using Western blot analysis. The cells were treated with LPS (0–10 μg/mL) for 24 and 48 hours. The expression of both COX-2 and IL-1β are increased according to cell damage or exposure duration. (B) The protein levels of Cu/Zn SOD and Mn SOD as an antioxidant enzyme were examined. The expressions were checked at 24 and 48 hours after LPS stress (10 μg/mL). Pretreated Ad/IGFBP-3 group maintained the levels of SOD enzyme. (C) Phospho-ERK and Phospho-p38MAPK signaling were measured. The adenoviral vector Ad/IGFBP-3 reduced the expression of P-p38MAPK and P-ERK. (D) The NF-κB activity was measured. The adenoviral vector Ad/IGFBP-3 reduced translocation of NF-κB from the cytosol to the nucleus. (E) Total cell lysate was measured using Western blot analysis. Pretreatment with Ad/IGFBP-3 reduced the expression of these pro-inflammatory cytokines (COX-2, IL-1β, TNF-α). (F) Treated with Ad/IGFBP-3 reduced the expression of phospho-IκB-α.

IEC-6 = intestinal epithelial cell 6, SOD = superoxide dismutase, LPS = lipopolysaccharides, IL = interleukin, COX = cyclooxygenase, Ad/IGFBP-3 = adenoviral vector system expressing insulin-like growth factor-binding protein-3, NF-κB = nuclear factor-κB, TNF-α = tumor necrosis factor-α.