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The qPCR assay efficiently amplifies cst-II, cst-III, and waaM. Serial 10-fold dilutions of control strains R4B202 (cst-II), R4B208 (cst-III), and R7B122 (cst-negative) were used as template in the multiplex qPCR assay to determine the PCR efficiency of the waaM (A) and cst-II/cst-III (B) targets.
