Fig. 3.

Immunoglobulin modulates dendritic cell activation profile a Gate strategy used for flow cytometry analysis, with CD11c chosen as a marker for dendritic cell subpopulation and combined with the markers in interest to assess costimulatory molecules expression on DCs surface. b Flow cytometry shows decreased expression of costimulatory molecules on activated DC surface (p = 0.0094 for MHC⁺; p = 0.0091 for CD86⁺ and p = 0.0213 for CD40) in IG treated compared to control mice, data are means ± SE of six animals per group (WT, dko control and dko IG) from three independent experiments (n = 2 mice per group in each one of them), totalizing n = 18 mice analyzed (*p < 0.05; **p < 0.01)