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. 2018 Mar 23;8:5142. doi: 10.1038/s41598-018-23379-2

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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(a) RNA-Seq analysis of up-regulated genes of interest in vehicle treated BeWo cells (−) or BeWo cells treated with forskolin (+) for the times described in Materials and Methods. Results are expressed in transcripts per million (TPM). Each bar represents the mean + /− SEM (n = 6). (b) Quantitative Western blot analysis of up-regulated genes of interest in vehicle treated BeWo cells (−) or BeWo cells treated with forskolin (+) for the times described in Materials and Methods. Immunoblots were quantified and normalized to levels of actin and subsequently to the 0h- sample of each blot.