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. 2018 Mar 23;8:5145. doi: 10.1038/s41598-018-23338-x

Figure 1.

Figure 1

CD9/CD81 DKO mice progressively exhibit aging phenotype with shorter survival. (a) Representative photo from WT mouse (lower) and DKO mice (upper) at 80 weeks of age, Whole body (left) and eyes (right) from each group. (b) Body weight (left: male; n = 3–6, right: female; n = 3–5). DKO mice exhibit infertility, as described previously. (c) Representative CT images of the body trunk of WT and DKO mice at 80 weeks of age. Note that DKO mice exhibit severe kyphosis. Bone mineral density (BMD) decreased with age (n = 2–4). (d,e) Quantitation of volume of muscle (n = 4–5) and adipose tissue by micro-CT (n = 2–4). Note the reduction in volume of muscle and adipose. Yellow: subcutaneous fat, Pink: visceral fat, Blue: muscle. (f) Gross necropsy of euthanized moribund mice revealed a cachectic appearance. Isolated thymus (upper), spleen (middle), and testis were smaller in DKO mice (left: WT, right: DKO). (g) Comparison of lifespans. Survival was analyzed by the Kaplan-Meier method and log-rank test. WT: male (n = 14), female (n = 24), DKO: male (n = 25), female (n = 19) Note that DKO mice of both sexes exhibit shorter survival. Bars represent means ± SD; *P < 0.05, **P < 0.01 versus WT. Scale bar, 10 mm for (c), 30 mm for (d) and (e) and 5 mm for (f).