Table 1.
Data collection and refinement statistics
| Fam20A−Fam20C | Fam20A−drFam20C | drFam20C | hmFam20 | hmFam20 with Gal-Xyl | |
|---|---|---|---|---|---|
| Data collection | |||||
| Space group | P 21 21 21 | P 41 21 2 | P 21 | P 21 21 21 | P 21 21 21 |
| Cell dimensions | a = 78.469 Å, | a = 110.059 Å, | a = 114.925 Å, | a = 80.221 Å, | a = 80.601 Å, |
| b = 192.747 Å, | b = 110.059 Å, | b = 135.988 Å, | b = 123.222 Å, | b = 123.580 Å, | |
| c = 226.669 Å | c = 461.698 Å | c = 219.971 Å; β = 90.495° | c = 144.393 Å | c = 143.936 Å | |
| Resolution (Å) | 3.30 | 3.55 | 3.45 | 2.20 | 2.85 |
| Rmerge | 0.160 (0.815) | 0.351 (1.665) | 0.235 (0.883) | 0.101 (0.995) | 0.216 (1.484) |
| R pim | 0.078 (0.415) | 0.109 (0.546) | 0.143 (0.543) | 0.029 (0.298) | 0.062 (0.445) |
| I/σI | 10.0 (2.25) | 11.5 (2.0) | 8.0 (2.2) | 28.8 (2.9) | 14.8 (2.0) |
| Completeness (%) | 99.7 (100.0) | 100.0 (100.0) | 99.5 (99.0) | 99.7 (95.3) | 99.8 (99.7) |
| Multiplicity | 5.0 (4.7) | 11.4 (10.5) | 3.7 (3.6) | 12.8 (10.9) | 12.8 (11.6) |
| Wilson B-factor | 57.0 | 62.0 | 60.1 | 30.6 | 52.2 |
| Refinement | |||||
| Reflections used in refinement | 47,610 (2769) | 31,137 (1357) | 88,731 (8732) | 67,332 (4770) | 32,120 (2363) |
| Reflections used for Rfree | 2159 (125) | 1556 (68) | 2003 (199) | 2000 (142) | 1606 (118) |
| Rwork/Rfree | 0.233/0.267 | 0.262/0.300 | 0.225/0.279 | 0.180/0.211 | 0.200/0.217 |
| No. of non-hydrogen atoms | |||||
| Protein | 12,888 | 13,508 | 37,919 | 5685 | 5665 |
| Ligands | 124 | 124 | 0 | 56 | 122 |
| Solvent | 0 | 0 | 0 | 545 | 0 |
| Protein residues | 1579 | 1655 | 4594 | 706 | 709 |
| B-factors | |||||
| Protein | 68.52 | 61.95 | 54.36 | 41.5 | 49.3 |
| Ligands | 82.38 | 72.14 | 90.2 | 79.9 | |
| Solvent | 44.4 | ||||
| R.m.s deviations | |||||
| Bond lengths (Å) | 0.004 | 0.003 | 0.004 | 0.007 | 0.004 |
| Bond angles (°) | 0.73 | 0.66 | 1.01 | 1.23 | 1.00 |
| Ramachandran | |||||
| Favored (%) | 89.61 | 90.23 | 88.10 | 96.8 | 96.7 |
| Allowed (%) | 10.01 | 9.41 | 10.69 | 3.2 | 3.2 |
| Outliers (%) | 0.39 | 0.37 | 1.21 | 0 | 0.1 |
Each dataset was collected from a single crystal. Values in parentheses are for highest-resolution shell