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. 2018 Mar 23;8:5084. doi: 10.1038/s41598-018-23110-1

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Demonstrative principal component analysis (PCA) score plots of FIE-MS data of 2285 normalised m/z intensity values (P < 0.05) in both negative and positive ionisation mode, 6 h after antibiotic treatment: (A) AMP-ampicillin, C-control group, EMB-ethambutol, ETH-ethionamide, INH-isoniazid, KAN-kanamycin, LIN-linezolid, PRE-pretomanid, RIF-rifampicin, STR-streptomycin; (B) samples were clustered based on their mechanism of action, CW-cell wall synthesis, PS-protein synthesis, PRE-pretomanid treatment. Coloured regions display 95% confidence.