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. 2018 Mar 23;9:1214. doi: 10.1038/s41467-018-03682-2

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — Neuronal characterization of different subpopulations activated in the discriminator rats following a new tone or CS presentation within the LA. a Representative photomicrograph of triple catFISH showing neurons expressing H1a(green arrows), Arc (red), and CamKIIa (white) mRNAs. The merged panel shows nuclei that were single-, double- or triple-labeled for H1a, Arc, and CamKIIa. Scale bar, 20 µm. b Dot-plots graphs showing the percentage of cells coexpressing either H1a (new tone) or Arc (CS) or H1a + Arc (new tone + CS) with CamKIIa. c Images of triple catFISH showing expression of H1a, Arc, and Thy-1 mRNAs. d The percentages of cells coexpressing Thy-1 and either H1a or Arc or those coexpressing H1a + Arc were low and similar among the three conditions. e Images showing the expression of Arc, H1a, and Grp mRNAs. f The percentages of cells coexpressing Grp and either H1a or Arc or both mRNAs were high and similar among the three conditions. g Photomicrographs showing neuronal expression of H1a, Arc, and Parvalbumin (PV) mRNAs. h The percentages of cells coexpressing PV were similar in neurons that were activated following the presentation of the new tone or the CS. i Images showing triple catFISH for Arc, H1a, and Somatostatin (SOM) mRNAs. j The percentages of cells coexpressing SOM with Arc, H1a, or H1a + Arc were similar following the presentation of the new tone or the CS. k Images of triple catFISH showing the mRNA expression of H1a, Arc, and Calretinin (CR). l There were no differences in the percentages of cells coexpressing CR with H1a, Arc, or H1a + Arc. m Pie charts summarizing the percentages of cells in different subpopulations of neurons that were activated by the new tone, CS or both, according to the expression of different neuronal markers. All data are mean and SEM