Fig. 2.
Assay performance evaluation in 384-well HTS format. (A) Transfection efficiency monitored by images of HEK 293T cells cotransfected (green) with VF-NSD3 and GST-MYC. Images were taken by ImageXpressmicro automated imaging system (Molecule Devices). (B) Z′ factor and S/B of the assay with increasing amount of cell lysate. (C) Temporal stability of TR-FRET signal. The TR-FRET signal with increasing amount of cell lysate was measured in over a time course of 96 h. (D) The TR-FRET signal in the presence of increasing amount of DMSO. DMSO, dimethyl sulfoxide; HTS, high-throughput screening. Color images available online at www.liebertpub.com/adt