Table 1.

Combinations of Constructs and Time-Resolved Fluorescence Resonance Energy Transfer Antibody Pairs

No.	NSD3 Constructs	MYC Constructs	S/B	TR-FRET Antibody Pair (Donor/Acceptor)
1	N-VF-NSD3	N-His-MYC	1.3	Anti-Flag-Tb/anti-His-d2
2	N-VF-NSD3	C-His-MYC	2.3
3	C-VF-NSD3	N-His-MYC	1.1
4	C-VF-NSD3	C-His-MYC	1.1
5	N-His-NSD3	N-VF-MYC	1.2
6	N-His-NSD3	C-VF-MYC	1.1
7	N-His-NSD3	N-Flag-MYC	1.1
8	N-His-NSD3	C-Flag-MYC	1.1
9	C-His-NSD3	N-VF-MYC	1.2
10	C-His-NSD3	C-VF-MYC	1.1
11	C-His-NSD3	N-Flag-MYC	0.9
12	C-His-NSD3	C-Flag-MYC	1.1
13	N-Flag-NSD3	N-His-MYC	1.0
14	N-Flag-NSD3	C-His-MYC	1.1
15	C-Flag-NSD3	N-His-MYC	1.1
16	C-Flag-NSD3	C-His-MYC	0.9
17	N-VF-NSD3	GST-MYC	13.4	Anti-Flag-Tb/anti-GST-d2
18	C-VF-NSD3	GST-MYC	1.0
19	N-Flag-NSD3	GST-MYC	1.1
20	C-Flag-NSD3	GST-MYC	1.0
21	GST-NSD3	N-VF-MYC	3.0
22	GST-NSD3	C-VF-MYC	2.8
23	GST-NSD3	N-Flag-MYC	1.1
24	GST-NSD3	C-Flag-MYC	6.3
25	GST-NSD3	N-His-MYC	1.0	Anti-His-Eu/anti-GST-d2
26	GST-NSD3	C-His-MYC	1.3
27	N-His-NSD3	GST-MYC	1.2
28	C-His-NSD3	GST-MYC	1.2
29	N-VF-NSD3	N-His-MYC	1.5	Anti-His-Tb/Venus
30	N-VF-NSD3	C-His-MYC	1.8
31	C-VF-NSD3	N-His-MYC	1.5
32	C-VF-NSD3	C-His-MYC	1.5
33	N-His-NSD3	N-VF-MYC	1.6
34	N-His-NSD3	C-VF-MYC	1.5
35	C-His-NSD3	N-VF-MYC	1.5
36	C-His-NSD3	C-VF-MYC	1.4
37	N-His-NSD3	GST-MYC	1.0	Anti-GST-Tb/anti-His-d2
38	C-His-NSD3	GST-MYC	1.2
39	GST-NSD3	N-His-MYC	1.0
40	GST-NSD3	C-His-MYC	1.1
41	N-VF-NSD3	GST-MYC	2.0	Anti-GST-Tb/Venus
42	C-VF-NSD3	GST-MYC	1.1
43	GST-NSD3	N-VF-MYC	6.7
44	GST-NSD3	C-VF-MYC	3.9
45	N-VF-NSD3	GST-MYC	2.1	Anti-GST-Tb/anti-Flag-APC
46	C-VF-NSD3	GST-MYC	1.2
47	N-Flag-NSD3	GST-MYC	1.3
48	C-Flag-NSD3	GST-MYC	1.0

APC, allophycocyanin; Eu, europium; GST, glutathione S-transferase; NSD3, nuclear receptor binding SET domain protein 3; S/B, signal-to-background; Tb, terbium; TR-FRET, time-resolved fluorescence resonance energy transfer; VF, Venus-Flag.