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. 2018 Mar 16;9:226. doi: 10.3389/fphar.2018.00226

FIGURE 1.

FIGURE 1

Effects of triptolide (TP), isoliquiritigenin (ISL), or ISL + TP on the viability and morphological changes of L02 cells. (A) L02 cells were treated with TP at various concentrations for 24 h. (B) L02 cells were treated with ISL at various concentrations for 6, 12, and 24 h. (C) L02 cells were pretreated with indicated ISL concentrations for 12 h followed by TP (50 nM, 24 h) treatment. (D) L02 cells were pretreated with 7.5 μM ISL for 12 h followed by TP (50 nM, 24 h) treatment. Cell morphological changes were detected by a light microscope (10 × magnification). The cell viability was determined by MTT assay. Data were presented as means ± standard error (SE) (n = 3); ∗∗∗P < 0.001 vs. the control group, #P < 0.05 vs. the TP group, and ###P < 0.001 vs. the TP group.