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. 2018 Mar 20;131(6):704–712. doi: 10.4103/0366-6999.226889

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Copyright: © 2018 Chinese Medical Journal

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 3.0 License, which allows others to remix, tweak, and build upon the work non-commercially, as long as the author is credited and the new creations are licensed under the identical terms.

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The quantification and characterization of exosomes. (a) BCA method was performed to quantify protein concentration. Protein concentration standard curve was shown. After calculation, the extraction of the protein concentration of exosomes was 5.54 μg/μl. (b) NTA evaluation shows small vesicles within the expected range of exosomes (40–200 nm) in the sample isolated from the ADSCs culture medium by ultracentrifugation. BCA: Bicinchoninic acid; NTA: Nanoparticle tracking analysis; ADSCs: Adipose-derived stem cells.