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. 2018 Mar 16;9:267. doi: 10.3389/fpls.2018.00267

Figure 1.

Figure 1

ipd3l ipd3-2 mutant is impaired in nodule development. (A) Phenotypic analyses of wild-type R108, ipd3l, ipd3-2, and ipd3l ipd3-2 double mutants at 4 weeks post-inoculation (wpi) with Sinorhizobium meliloti (strain 1021). Pink nodules were developed on R108 and ipd3l roots. Uninfected nodules (bump) were found on ipd3-2 roots. No pink nodules or bumps were observed on the ipd3l ipd3-2 double mutant roots. (B) Quantitative analysis of nodules grown on R108, ipd3l, ipd3-2, and ipd3l ipd3-2 roots. (C) Representative images of infection threads in the wild-type, ipd3l, ipd3-2 and ipd3l ipd3-2 mutants. S. meliloti 1021 is labeled by green fluorescent protein (GFP). (D) Quantitative analysis of infection threads (IT) grown on R108, ipd3l, ipd3-2, and ipd3l ipd3-2 roots. Scale bars correspond to 1 mm in (A,B) 100 μm in (C). These experiments were repeated three times with similar results. The error bars indicate standard error. The asterisk indicates a significant decrease relative to the control with Student's t-test (*P ≤ 0.01).