Figure 2.
Expression pattern of IPD3 and IPD3L. (A) GUS activity of the pIPD3:GUS and pIPD3L:GUS transformed plants at different time points with or without inoculation by S. meliloti 1021. The left panel shows the whole root system. These experiments were repeated more than three times. (B) Expression analyses of IPD3 by real-time PCR at 1, 2, 4 wpi. (C) Expression analyses of IPD3L by real-time PCR at 1, 2, 4 wpi. Scale bars in the left panel correspond to 5 cm; other scale bars correspond to 100 μm. These experiments were repeated three times. More than 10 plants were used for RNA extraction. The error bars indicate standard error (n = 3). The asterisk indicates a significant decrease relative to the control (−Sm1021) with Student's t-test (*P ≤ 0.01).