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. 2018 Mar 16;9:267. doi: 10.3389/fpls.2018.00267

Figure 5.

Figure 5

IPD3-2D triggers spontaneous root-like nodules. (A) Type I spontaneous nodules developed on the ipd3l ipd3-2 hairy roots transformed with the phosphomimetic IPD3-2D mutant version, Type II and III developed on the hairy roots of dmi3-1 and dmi2-1 transformed with the phosphomimetic IPD3-2D mutant version. Spontaneous nodules were observed and scored at 8 weeks transformed into the soil. (B) Toluidine blue-stained section of a pink nodule induced by S. meliloti 1021. (C) Toluidine blue-stained sections of type I spontaneous root-like nodules. (D) Toluidine blue-stained sections of type II spontaneous root-like nodules. These experiments were repeated three times with similar results. Scale bars in (A) correspond to 1 mm; scale bars in (B–D) correspond to 0.1 mm.