Fig. 6.

TLR3 inhibitor attenuates cocaine-induced upregulation of NF-κB signaling. Phospho-NF-κB p65 in the cytoplasm (a) and nucleus (b) in the NAc are significantly increased after cocaine CPP. IKKβ (c) and phosphorylated IκBα (d) are upregulated. *p < 0.05, **p < 0.01, n = 3/saline group, n = 3/cocaine group. Intra-NAc Inhibitor (50 μM, 1 μl/injection) decreased cocaine CPP-induced the expressions of nuclear phospho-NF-κB p65 (e), cytoplasmic IKKβ (f) and phosphorylated IκBα (g) in the NAc. *p < 0.05, **p < 0.01, n.s Not significant. n = 4/vehicle+saline group, n = 4/inhibitor+saline group, n = 4/vehicle+cocaine group, n = 4/inhibitor+cocaine group. Data are presented as the mean ± SEM