Fig. 4.

Synergistic effects between the crude drugs found in UKT according to the yeast two-hybrid assay. a The synergistic effects of combining glycyrrhizae radix with other crude drugs on ERβ-dependent estrogenic activity. b The synergistic effects of combining evodiae fructus with other crude drugs on ERβ-dependent estrogenic activity. The β-galactosidase activity of the crude drugs alone and various pairs of crude drugs were evaluated at concentrations of 0.1 mg/ml (black columns). **significantly increased compared with the sum of the activity levels of the two crude drugs alone, p < 0.01. SQ values were calculated to determine whether each combination had synergistic effects, as described in the materials and methods. The data for the combinations that did not have significant synergistic effects in the yeast two-hybrid assay are not shown