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. 2018 Mar 22;18:317. doi: 10.1186/s12885-018-4221-0

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© The Author(s). 2018

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 5 — Reduced DNA methylation of DMRs of Mst1r, Slpi, and Extl1 after 5-aza-dC treatment are associated with up-regulation of these genes in hepa1c1c7 cells and Hepa1-6 cells. Hepa1-6 cells (b, d, e) and Hepa1c1c7 cells (a, c) were cultured with 0, 0.1 or 1 μM of 5-aza-dC, and 0, 50 or 100 μM of 5-aza-dC for 72 h, respectively. Left figures: the results of bisulfite sequencing of CpGs detected in the DMRs of liver tumors in C3H mice. ●: methylated cytosine, ○: unmethylated cytosine. Right figures: the expressions of Mst1r, Slpi, and Extl were measured by real-time PCR and normalized to the expression of β-actin or rRNA (n = 3). Statistical significance was analyzed by one-way ANOVA followed by Turkey-Kramer test as a post hoc comparison. *^, **^, *** Significantly different at p < 0.05, 0.01, and 0.001, respectively