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. 2018 Jan 11;103(4):707–716. doi: 10.3324/haematol.2017.167486

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Figure 3. — Pro-survival signals are delivered to multiple myeloma (MM) cell lines. (A) Western blot analyses of pAkt, Akt, survivin and actin in 3D versus 2D co-cultures of HS-5 and RPMI.8266 or MM1.S cells and of primary bone marrow stromal cells (BMSC) and MM1.S cells (one representative experiment out of three) and also on isolated HS-5 cells. (B) Mean±Standard Error of Mean (SEM) of pAkt/totalAkt ratios from three independent experiments. β2-microglobulin (C), IL-6, angiopoietin-2 (Ang-2), VEGF and FGF (D) levels in 3D culture supernatants. *P≤0.05.