Figure 7.

3C12C reduced B cells in non-human primates. Five nonhuman primates were injected with 3C12C (1, 5, 10, 10 mg/kg, n=4) or human Immunoglobulin G (IgG; 10mg/kg, n=1) at days 0, 7, 14 and 21. Blood and serum samples were collected for cell counts (red cells, white cells and platelets), liver function (ALP and AST levels) and kidney function (creatinine level) analysis. (A) CD19⁺ B cells were enumerated from PBMC of five animals by flow cytometry. Dashed lines indicate the base cell number at day 0. *indicates one time point when WBC was extremely high on that animal. (B) A lymph node biopsy was taken at day 28 from 3C12C (10mg/kg) and control-treated animals. B cells stained with anti-human CD20 mAb on paraffin-embedded lymph node biopsy samples are shown. One of the two similar results for the two animals receiving 10 mg/kg 3C12C showing reduced B-cell areas compared to the human IgG control animal.