Figure 2.

Labile iron pool (LIP) status in MCF-7 cells following treatment with increasing concentrations of deferoxamine (DFO). The effect of iron chelation dose on intracellular iron content was assessed in untreated cells and cells treated with DFO by comparing calcein acetoxymethyl ester (CA-AM)-stained cells with that of CA-AM + chelator-stained cells as explained in Methods section. Average mean fluorescence intensity (MFI) ± standard error of the mean (SEM) of histograms obtained from 2 separate experiments for cells treated with 1, 5, or 10 μM DFO for 24 hours (A) and 48 hours (B) and from 3 separate experiments for cells treated with 30, 100, or 300 μM DFO for 24 hours (C) and 48 hours (D). E, Average change MFI (▵MFI) ± SEM in control and treated cells at 24 and 48 hours as calculated by the formula given in Methods section. P > .05 signifies the presence of a statistically significant difference in ▵MFI (E) in the specific control or experimental group.