Table 2.
The differential proteins in the reversible (Rev) and irreversible (Irr) CHD-PAH groups compared with the control (Con) group.
| Protein name | iTRAQ-ratio Rev:Con | iTRAQ-ratio Irr:Con | iTRAQ-ratio Irr:Rev | P value Rev:Con | P value Irr:Con | P value Irr:Rev |
|---|---|---|---|---|---|---|
| The upregulated proteins | ||||||
| Collagen alpha-1(VI) chain | 0.809 | 3.908 | 4.966 | 0.268 | 0.008 | 0.000 |
| Collagen alpha-1(XII) chain | 0.570 | 2.535 | 4.406 | 0.021 | 0.000 | 0.000 |
| Cathepsin D | 1.180 | 4.742 | 3.698 | 0.295 | 0.001 | 0.000 |
| Sushi domain-containing protein 2 | 0.731 | 2.805 | 3.597 | 0.509 | 0.001 | 0.000 |
| Sodium/potassium-transporting ATPase subunit alpha-1 | 1.542 | 5.395 | 3.532 | 0.129 | 0.000 | 0.000 |
| Basement membrane-specific heparan sulfate proteoglycan core protein | 1.380 | 4.446 | 3.373 | 0.219 | 0.000 | 0.000 |
| Myosin-14 | 1.000 | 3.251 | 3.251 | 0.364 | 0.000 | 0.000 |
| Voltage-dependent-anion-selective channel protein 2 | 0.738 | 2.27 | 3.162 | 0.940 | 0.024 | 0.009 |
| Keratin, type II cytoskeletal 7 | 0.581 | 1.888 | 3.133 | 0.048 | 0.014 | 0.000 |
| LIM and calponin homology domains-containing protein 1 | 0.724 | 2.148 | 2.965 | 0.422 | 0.037 | 0.000 |
| Collagen alpha-6(VI) chain | 1.459 | 4.130 | 2.884 | 0.079 | 0.000 | 0.000 |
| Collagen alpha-2(IV) chain | 1.067 | 2.938 | 2.884 | 0.272 | 0.003 | 0.000 |
| Phosphoglucomutase-like protein 5 | 1.047 | 2.831 | 2.884 | 0.433 | 0.018 | 0.000 |
| Platelet glycoprotein 4 | 1.086 | 2.992 | 2.78 | 0.762 | 0.003 | 0.004 |
| Vinculin | 1.127 | 3.076 | 2.754 | 0.681 | 0.000 | 0.000 |
| Matrix metalloproteinase-9 | 0.679 | 2.032 | 2.729 | 0.330 | 0.021 | 0.002 |
| Desmin | 1.770 | 4.742 | 2.704 | 0.278 | 0.000 | 0.000 |
| Filamin-B | 0.973 | 2.630 | 2.679 | 0.957 | 0.000 | 0.000 |
| Alpha-actinin-4 | 1.096 | 2.884 | 2.655 | 0.385 | 0.003 | 0.000 |
| Myosin-9 | 2.399 | 5.808 | 2.466 | 0.088 | 0.000 | 0.000 |
| Transgelin | 0.938 | 2.312 | 2.466 | 0.592 | 0.008 | 0.000 |
| Prenylcysteine oxidase 1 | 1.66 | 4.093 | 2.466 | 0.010 | 0.000 | 0.000 |
| Lactadherin | 3.133 | 7.047 | 2.377 | 0.016 | 0.000 | 0.006 |
| Chondroitin sulfate proteoglycan 4 | 1.393 | 3.311 | 2.355 | 0.606 | 0.012 | 0.013 |
| Tropomyosin beta chain | 1.570 | 3.532 | 2.355 | 0.947 | 0.025 | 0.001 |
| Myosin light polypeptide 6 | 2.168 | 4.875 | 2.333 | 0.429 | 0.020 | 0.019 |
| Myeloperoxidase | 0.887 | 2.051 | 2.312 | 0.385 | 0.028 | 0.000 |
| Filamin-A | 2.032 | 4.656 | 2.291 | 0.015 | 0.000 | 0.000 |
| Alpha-actinin-1 | 1.009 | 2.270 | 2.270 | 0.475 | 0.000 | 0.000 |
| Laminin subunit beta-2 | 2.377 | 5.297 | 2.208 | 0.110 | 0.000 | 0.000 |
| Fibulin-5 | 2.188 | 4.656 | 2.208 | 0.148 | 0.000 | 0.004 |
| Laminin subunit alpha-5 | 2.148 | 4.487 | 2.188 | 0.064 | 0.000 | 0.000 |
| Platelet endothelial cell adhesion molecule | 2.679 | 5.754 | 2.188 | 0.028 | 0.000 | 0.001 |
| Glutamyl aminopeptidase | 3.945 | 8.551 | 2.168 | 0.030 | 0.000 | 0.001 |
| Myosin-11 | 2.466 | 5.297 | 2.148 | 0.000 | 0.000 | 0.000 |
| Intercellular adhesion molecule 1 | 1.067 | 2.270 | 2.128 | 0.658 | 0.000 | 0.000 |
| Tropomyosin alpha-1 chain | 2.249 | 4.699 | 2.109 | 0.305 | 0.000 | 0.000 |
| Calreticulin | 1.380 | 2.884 | 2.109 | 0.494 | 0.000 | 0.000 |
| Proteasome subunit alpha type-6 | 0.929 | 2.032 | 2.109 | 0.765 | 0.033 | 0.008 |
| Spectrin beta chain, brain 1 | 3.133 | 6.486 | 2.089 | 0.000 | 0.000 | 0.000 |
| Annexin A4 | 0.847 | 1.803 | 2.070 | 0.722 | 0.001 | 0.002 |
| Hemicentin-1 | 1.393 | 2.754 | 2.051 | 0.568 | 0.002 | 0.001 |
| Cell surface glycoprotein MUC18 | 1.445 | 2.858 | 2.014 | 0.652 | 0.013 | 0.017 |
| Integrin alpha-2 | 1.803 | 3.404 | 1.995 | 0.074 | 0.003 | 0.041 |
| Spectrin alpha chain | 2.421 | 4.786 | 1.977 | 0.001 | 0.000 | 0.000 |
| Myosin-10 | 3.076 | 5.916 | 1.923 | 0.010 | 0.000 | 0.000 |
| Calnexin | 2.606 | 4.966 | 1.923 | 0.008 | 0.000 | 0.008 |
| Transmembrane protein 43 | 1.445 | 2.754 | 1.923 | 0.496 | 0.003 | 0.009 |
| Acid ceramidase | 1.432 | 2.655 | 1.923 | 0.399 | 0.008 | 0.005 |
| Sorbin and SH3 domain-containing protein 2 | 1.117 | 2.109 | 1.923 | 0.839 | 0.006 | 0.009 |
| Band 4.1-like protein 2 | 2.014 | 4.406 | 1.905 | 0.458 | 0.001 | 0.001 |
| Laminin subunit gamma-2 | 1.368 | 2.559 | 1.888 | 0.321 | 0.000 | 0.000 |
| Sulfide:quinone oxidoreductase, mitochondrial | 0.964 | 1.854 | 1.888 | 0.791 | 0.002 | 0.001 |
| Caldesmon | 1.282 | 2.377 | 1.871 | 0.131 | 0.000 | 0.000 |
| Palmitoyl-protein thioesterase 1 | 1.419 | 2.512 | 1.854 | 0.433 | 0.003 | 0.011 |
| Caveolin-1 | 2.535 | 4.613 | 1.837 | 0.126 | 0.006 | 0.014 |
| Membrane primary amine oxidase | 1.343 | 2.421 | 1.820 | 0.310 | 0.001 | 0.000 |
| Tryptase alpha/beta-1 | 1.837 | 3.281 | 1.803 | 0.729 | 0.001 | 0.002 |
| Basal cell adhesion molecule | 1.754 | 3.105 | 1.803 | 0.033 | 0.000 | 0.005 |
| Laminin subunit gamma-1 | 1.754 | 3.133 | 1.786 | 0.011 | 0.000 | 0.000 |
| Filamin-C | 1.528 | 2.679 | 1.786 | 0.285 | 0.000 | 0.000 |
| Erlin-2 | 1.380 | 2.399 | 1.786 | 0.640 | 0.009 | 0.023 |
| Synemin | 1.528 | 2.606 | 1.770 | 0.501 | 0.002 | 0.005 |
| Fibulin-1 | 1.117 | 1.959 | 1.770 | 0.992 | 0.007 | 0.005 |
| Cadherin-5 | 2.148 | 3.767 | 1.770 | 0.007 | 0.000 | 0.010 |
| Early endosome antigen 1 | 0.912 | 1.644 | 1.754 | 0.687 | 0.001 | 0.000 |
| Collagen alpha-1(XVIII) chain | 1.486 | 2.559 | 1.722 | 0.002 | 0.000 | 0.002 |
| Periaxin | 1.754 | 2.992 | 1.690 | 0.166 | 0.000 | 0.000 |
| Hexokinase-1 | 1.472 | 2.443 | 1.690 | 0.381 | 0.002 | 0.023 |
| Myosin phosphatase Rho-interacting protein | 1.159 | 1.923 | 1.690 | 0.534 | 0.015 | 0.039 |
| Laminin subunit alpha-3 | 2.443 | 4.130 | 1.675 | 0.260 | 0.000 | 0.000 |
| Myoferlin | 1.107 | 1.786 | 1.660 | 0.779 | 0.003 | 0.001 |
| von Willebrand factor | 1.247 | 2.014 | 1.660 | 0.277 | 0.001 | 0.007 |
| Integrin beta-1 | 2.109 | 3.436 | 1.660 | 0.032 | 0.001 | 0.023 |
| Nestin | 1.854 | 3.048 | 1.644 | 0.014 | 0.000 | 0.000 |
| Protein disulfide-isomerase | 1.528 | 2.466 | 1.629 | 0.568 | 0.001 | 0.002 |
| Sorbin and SH3 domain-containing protein 1 | 1.138 | 1.837 | 1.629 | 0.561 | 0.004 | 0.004 |
| Palladin | 1.629 | 2.559 | 1.600 | 0.631 | 0.028 | 0.008 |
| Nidogen-1 | 3.565 | 5.495 | 1.556 | 0.000 | 0.000 | 0.001 |
| Integrin alpha-1 | 3.281 | 5.058 | 1.556 | 0.000 | 0.000 | 0.009 |
| Epoxide hydrolase 1 | 1.690 | 2.582 | 1.556 | 0.003 | 0.000 | 0.013 |
| Cytoplasmic FMR1-interacting protein 1 | 4.055 | 6.310 | 1.528 | 0.182 | 0.039 | 0.043 |
| Glutamatedehydrogenase1,mitochondrial | 1.247 | 1.854 | 1.514 | 0.251 | 0.001 | 0.006 |
| Afadin | 1.159 | 1.738 | 1.514 | 0.930 | 0.007 | 0.006 |
| Lysozyme C | 1.786 | 2.655 | 1.500 | 0.745 | 0.034 | 0.047 |
| The downregulated proteins | ||||||
| Carbonic anhydrase 1 | 0.802 | 0.540 | 0.667 | 0.226 | 0.001 | 0.006 |
| Stress-induced-phosphoprotein 1 | 0.731 | 0.497 | 0.655 | 0.267 | 0.001 | 0.007 |
| Sorcin | 0.759 | 0.474 | 0.614 | 0.382 | 0.006 | 0.019 |
| Cytosolic non-specific dipeptidase | 0.711 | 0.429 | 0.603 | 0.359 | 0.006 | 0.035 |
| 60S ribosomal protein L5 | 0.973 | 0.560 | 0.597 | 0.570 | 0.011 | 0.039 |
| Leucine-rich alpha-2-glycoprotein | 0.597 | 0.347 | 0.575 | 0.029 | 0.001 | 0.020 |
| Antithrombin-III | 1.028 | 0.555 | 0.530 | 0.258 | 0.013 | 0.001 |
| Serine/arginine-rich splicing factor 9 | 0.991 | 0.511 | 0.525 | 0.914 | 0.039 | 0.040 |
| Ubiquitin-like modifier-activating enzyme 1 | 1.180 | 0.614 | 0.520 | 0.307 | 0.000 | 0.000 |
| Fructose-1,6-bisphosphatase 1 | 1.169 | 0.570 | 0.515 | 0.993 | 0.024 | 0.021 |
| Vacuolar protein sorting-associated protein 4B | 0.501 | 0.278 | 0.511 | 0.062 | 0.005 | 0.035 |
| Apolipoprotein B-100 | 1.009 | 0.501 | 0.506 | 0.368 | 0.000 | 0.000 |
| Glyceraldehyde-3-phosphate dehydrogenase | 0.855 | 0.429 | 0.506 | 0.233 | 0.004 | 0.011 |
| Elongation factor 2 | 0.802 | 0.437 | 0.501 | 0.156 | 0.004 | 0.015 |
| Ig gamma-2 chain C region | 0.964 | 0.461 | 0.497 | 0.357 | 0.017 | 0.033 |
| Ceruloplasmin | 0.692 | 0.344 | 0.479 | 0.077 | 0.000 | 0.007 |
| Catalase | 0.904 | 0.461 | 0.466 | 0.387 | 0.012 | 0.006 |
| Bifunctional purine biosynthesis protein PURH | 0.920 | 0.413 | 0.457 | 0.290 | 0.002 | 0.009 |
| SAM domain and HD domain-containing protein 1 | 1.138 | 0.497 | 0.453 | 0.906 | 0.008 | 0.005 |
| Isoleucine–tRNA ligase, cytoplasmic | 1.127 | 0.506 | 0.453 | 0.880 | 0.043 | 0.011 |
| Plasma cell-induced resident endoplasmic reticulum protein | 0.832 | 0.366 | 0.449 | 0.460 | 0.018 | 0.038 |
| 40S ribosomal protein S4, X isoform | 0.631 | 0.291 | 0.445 | 0.041 | 0.000 | 0.043 |
| Aflatoxin B1 aldehyde reductase member 2 | 0.759 | 0.319 | 0.425 | 0.076 | 0.002 | 0.021 |
| 40S ribosomal protein S13 | 0.780 | 0.319 | 0.413 | 0.469 | 0.015 | 0.016 |
| Keratin, type I cytoskeletal 17 | 0.692 | 0.308 | 0.413 | 0.153 | 0.005 | 0.027 |
| 60S ribosomal protein L18 | 1.213 | 0.470 | 0.413 | 0.945 | 0.020 | 0.022 |
| Plasma protease C1 inhibitor | 0.863 | 0.350 | 0.409 | 0.289 | 0.000 | 0.000 |
| Flavin reductase (NADPH) | 0.991 | 0.370 | 0.384 | 0.615 | 0.010 | 0.000 |
| Macrophage migration inhibitory factor | 0.497 | 0.175 | 0.380 | 0.021 | 0.024 | 0.008 |
| Histone H1.4 OS = Homo sapiens | 0.895 | 0.322 | 0.366 | 0.693 | 0.013 | 0.044 |
| Pyruvate kinase isozymes M1/M2 | 0.429 | 0.167 | 0.350 | 0.003 | 0.000 | 0.013 |
| Heterogeneous nuclear ribonucleoprotein U | 0.991 | 0.398 | 0.347 | 0.484 | 0.016 | 0.002 |
| Alcohol dehydrogenase [NADP(+)] | 0.787 | 0.254 | 0.334 | 0.053 | 0.000 | 0.005 |
| 60S ribosomal protein L10 | 0.406 | 0.136 | 0.319 | 0.003 | 0.000 | 0.038 |
| Leucine-rich repeat-containing protein 59 | 0.780 | 0.251 | 0.313 | 0.412 | 0.014 | 0.036 |
| T-complex protein 1 subunit gamma | 0.879 | 0.268 | 0.308 | 0.232 | 0.001 | 0.004 |
| Ig kappa chain V-IV region JI | 0.938 | 0.288 | 0.305 | 0.749 | 0.033 | 0.042 |
| 6-phosphogluconate dehydrogenase, decarboxylating | 0.938 | 0.296 | 0.296 | 0.609 | 0.002 | 0.010 |
| 60S ribosomal protein L4 | 1.117 | 0.337 | 0.294 | 0.555 | 0.021 | 0.001 |
| Malate dehydrogenase, cytoplasmic | 0.555 | 0.167 | 0.291 | 0.007 | 0.000 | 0.000 |
| Hemopexin | 0.555 | 0.151 | 0.273 | 0.002 | 0.000 | 0.001 |
| Adenylate kinase isoenzyme 1 | 0.570 | 0.149 | 0.247 | 0.141 | 0.002 | 0.012 |
| Annexin A5 | 0.904 | 0.219 | 0.236 | 0.317 | 0.013 | 0.000 |
| Vitamin D-binding protein | 0.570 | 0.141 | 0.233 | 0.061 | 0.003 | 0.005 |
| Adenylate kinase 2, mitochondrial | 0.991 | 0.229 | 0.231 | 0.078 | 0.000 | 0.005 |
| Glutathione S-transferase P | 0.649 | 0.153 | 0.229 | 0.744 | 0.038 | 0.017 |
| Ig kappa chain C region | 0.895 | 0.187 | 0.209 | 0.835 | 0.015 | 0.031 |
| Complement C3 | 0.773 | 0.171 | 0.200 | 0.000 | 0.000 | 0.000 |
| Apolipoprotein A-IV | 0.982 | 0.200 | 0.198 | 0.659 | 0.001 | 0.003 |
| Phenylalanine–tRNA ligase alpha subunit | 0.773 | 0.157 | 0.189 | 0.057 | 0.005 | 0.011 |
| Alpha-2-macroglobulin | 0.479 | 0.094 | 0.187 | 0.000 | 0.000 | 0.000 |
| Phosphatidylethanolamine-binding protein 1 | 0.929 | 0.172 | 0.187 | 0.218 | 0.000 | 0.000 |
| Complement C4-B | 0.278 | 0.063 | 0.185 | 0.000 | 0.000 | 0.001 |
| Alpha-2-HS-glycoprotein | 0.592 | 0.109 | 0.182 | 0.124 | 0.000 | 0.000 |
| Inter-alpha-trypsin inhibitor heavy chain H4 | 0.809 | 0.167 | 0.180 | 0.532 | 0.026 | 0.003 |
| Immunoglobulin J chain | 0.619 | 0.103 | 0.160 | 0.267 | 0.002 | 0.004 |
| Calcyphosin | 0.964 | 0.150 | 0.154 | 0.646 | 0.001 | 0.001 |
| 60S ribosomal protein L6 | 1.117 | 0.166 | 0.153 | 0.745 | 0.029 | 0.018 |
| 40S ribosomal protein S17-like | 0.592 | 0.091 | 0.149 | 0.158 | 0.019 | 0.039 |
| Apolipoprotein A-I | 0.619 | 0.091 | 0.142 | 0.005 | 0.000 | 0.000 |
| Alpha-1-acid glycoprotein 1 | 0.461 | 0.070 | 0.137 | 0.258 | 0.000 | 0.034 |
| Glyoxalase domain-containing protein 4 | 1.282 | 0.185 | 0.134 | 0.904 | 0.014 | 0.005 |
| 40S ribosomal protein S19 | 0.619 | 0.090 | 0.129 | 0.539 | 0.003 | 0.003 |
| Heparin cofactor 2 | 0.570 | 0.072 | 0.125 | 0.031 | 0.000 | 0.013 |
| Transthyretin | 0.492 | 0.050 | 0.101 | 0.059 | 0.001 | 0.005 |
| Polymeric immunoglobulin receptor | 2.228 | 0.209 | 0.096 | 0.001 | 0.022 | 0.000 |
| Hemoglobin subunit delta | 1.009 | 0.096 | 0.093 | 0.769 | 0.013 | 0.025 |
| Haptoglobin | 0.724 | 0.066 | 0.087 | 0.129 | 0.000 | 0.000 |
| 60S ribosomal protein L13 | 0.429 | 0.041 | 0.083 | 0.224 | 0.001 | 0.046 |
| Apolipoprotein A-II | 0.441 | 0.039 | 0.082 | 0.140 | 0.003 | 0.040 |
| Alpha-1-antitrypsin | 0.592 | 0.054 | 0.080 | 0.003 | 0.000 | 0.000 |
| Inter-alpha-trypsin inhibitor heavy chain H2 | 0.649 | 0.052 | 0.075 | 0.032 | 0.000 | 0.000 |
| Ig alpha-1 chain C region | 1.225 | 0.09 | 0.073 | 0.885 | 0.012 | 0.013 |
| Serotransferrin | 0.457 | 0.035 | 0.065 | 0.000 | 0.000 | 0.000 |
| Glutathione S-transferase Mu 1 | 0.631 | 0.023 | 0.033 | 0.251 | 0.005 | 0.006 |