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. 2017 Dec 14;6:401. doi: 10.1038/s41389-017-0003-9

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — High levels of MKP1 correlate with GSCs differentiation. a Analysis of the MKP1 mRNA expression levels in GSC lines grown in stem medium compared to differentiation conditions (n = 3). b MKP1 mRNA expression by RNA-seq analysis in G26 and NS-1 cells at 8, 32, and 64-day time course of BMP treatment after GF withdrawal (GF). Error bars denote SD of four independent experiments. c Frequency of MKP1 promoter methylation after BMP4 continous treatment at the indicated time points. d mRNA expression of MKP1 was analyzed in U87 and GNS166 lines under increasing 5-AZA concentrations. e Analysis of MKP1 mRNA expression levels in U251 cells with knockdown of SOX2 (shSOX2) or SOX9 (shSOX9) related to the control (pLKO) condition (n = 3). qRT-PCR data are normalized to GAPDH expression. f MKP1 levels in U87 cells with SOX2 or SOX9 overexpression compared to control cells (n = 3). g Association analysis of MKP1 with SOX2 and SOX9 in the human glioblastoma TCGA cohort from R2: Genomics Analysis and Visualization Platform http://r2.amc.nl. The analysis showed a negative correlation between MKP1 and SOX2 (R −0.207, p value 1.3e−06), and MKP1 and SOX9 (R −0.169, p value 1.7e−04)