Fig. 4.

MKP1 suppresses proliferation and self-renewal ability of GSCs. a mRNA levels of MKP1 in GNS166 cells infected with the indicated conditions (n = 3). b Cell growth assay comparing control and MKP1-overexpressing GSCs (n = 3). c Representative image and quantification of P-H3⁺ cells in both control and MKP1-overexpressing GNS166 cells (n = 3). d SOX2, SOX9, OCT4, and GFAP mRNA expression levels in GNS166 with ectopic activation of MKP1 relative to controls (n = 3). e Representative immunoblots of SOX2 and SOX9 derived from two different and independent retroviral infections. f Expression of TUJ1 and CNPase in GNS166 with ectopic MKP1 relative to control transduced cells (n = 3). g Representative images of TUJ1⁺- and CNPase⁺-infected GNS166 cells (n = 3) after 7 days cultured under differentiation conditions. h Control and MKP1 GNS166 cells were injected stereotaxically in nude mice (n = 5 and 3) and growth of the tumors was scored 120 days after