Fig. 6.

High levels of MKP1 sensitize glioma cells to temozolomide treatment. a mRNA expression of MKP1 was studied in U87 and U251 GBM cell lines under the effect of TMZ (100 μM) for 24 h. b Cell viability comparing control and MKP1-overexpressing U87 cells under 100 μM TMZ treatment for 72 h. c Cell viability comparing U87 cells transfected with shSC or shMKP1 cultured with 100 μM TMZ for 72 h. d Cell proliferation comparing control and MKP1-overexpressing GNS166 cells under 100 μM TMZ treatment. e Relative oncosphere-forming capacity of indicated U87 cells in presence of 50 μM TMZ for 7 days. f MGMT mRNA levels were assayed in both control and MKP1-transduced GNS166 and U87 cells. Statistical significance was obtained with Student’s t test (*p ≤ 0.05; **p ≤ 0.01; ***p ≤ 0.001)