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. 2018 Mar 19;13:1653–1664. doi: 10.2147/IJN.S159404

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© 2018 Dabrowska et al. This work is published and licensed by Dove Medical Press Limited

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The SR-SIM analysis of hBM-MSCs, 24 hours after their co-culture with EVs previously stained with different dyes.

Notes: EVs labeled with PKH26 (A–C) or tagged with Molday ION (D–F) (red) taken up by hBM-MSCs are visible inside the cells. Coexpression of tetraspanins: CD9 (A and D), CD63 (B and E), and CD81 (C and F) (green) were demonstrated. Cell nuclei were stained with Hoechst (blue). Scale bar =20 μm.

Abbreviations: EVs, extracellular vesicles; hBM-MSCs, human bone marrow mesenchymal stem cells; SR-SIM, super-resolution structured illumination microscopy.