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. 2018 Feb 1;7(1):10–24. doi: 10.1089/biores.2018.0008

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© Tina P. Dale and Nicholas R. Forsyth 2018; Published by Mary Ann Liebert, Inc.

This Open Access article is distributed under the terms of the Creative Commons License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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FIG. 1. — PChM supports stability and promotes increased pellet size. (A) μCT scans postfixation of day 1 and 20 pellets in MM or PChM indicate that pellets are formed by flattened, multilayered cell sheets folding or rolling to form spheroids. (B) Fold change in pellet volume relative to day 1. (C) Pellet volume at day 20. (D) Fold change in pellet density relative to day 1. (E) Pellet density at day 20. PD level for cells was as follows: OK3 PD6, OK3H PD50, BMA13 PD1, BMA13H PD16, 1C6 PD36, and 1C6H PD107. **p < 0.01, ***p < 0.001, ****p < 0.0001. Y-axis on (B) and (D) indicate fold change at day 20 relative to day 1, (C) pellet volume (mm³), (E) pellet density (relative units). Data are plotted as mean ± standard deviation, n = 3. μCT, microcomputed tomography; MM, maintenance medium; PChM, prochondrogenic medium; PD, population doubling.