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. Author manuscript; available in PMC: 2018 Nov 15.
Published in final edited form as: J Am Chem Soc. 2017 Nov 1;139(45):16222–16227. doi: 10.1021/jacs.7b07603

Figure 3.

Figure 3

Mass spectrometry analysis of UV-dependent HDAC8-interacting proteins. (A) Analysis of –UV and + UV enrichment for each HDAC8 Bpa mutant. Colored data points in the greyed quadrant were specifically enriched (p ≤ 0.05, ratio ≥ 2.5, peptides / protein ≥ 2) and assigned as putative UV-dependent interaction partners. (B) Venn diagram comparing the enrichment of candidate crosslinked proteins shows little overlap between different sites of incorporation. (C) Sequence alignment of predicted acetylated sites enriched by HDAC8 covalent substrate capture reveals preference for aromatic residues.