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. 2018 Jan 3;9(18):14124–14137. doi: 10.18632/oncotarget.23897

Figure 5. JNJ sensitizes naïve MCF7 cells by inhibiting AP-1 signal pathway and downregulated expression of XIAP.

Figure 5

(A) The temporal RNA expression of XIAP was measured by quantitative real time PCR in naïve MCF7 cells after JNJ treatment. (B) The temporal protein expression of XIAP was measured by western blot in naïve MCF7 cells after JNJ treatment. (C) The temporal RNA expression of c-Fos and c-Jun were measured by quantitative real time PCR in naïve MCF7 cells after JNJ treatment. (D) The temporal protein expression of c-Fos and c-Jun were measured by western blot in naïve MCF7 cells after JNJ treatment. (E) AP-1 expression was downregulated in nuclear in naïve MCF7 cells after JNJ treatment. (F) Localization and expression of AP-1 was measured by fluorescent immunohistochemistry assay in naïve MCF7 cells after JNJ treatment. (G) Relative binding activity of AP-1 to XIAP promoter regions was measured by CHIP assay in naïve MCF7 cells after JNJ treatment.