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. 2018 Feb 12;9(18):14228–14250. doi: 10.18632/oncotarget.24479

Table 1. Surface Expression of TRAIL-R1 and -R2 in NPC cells in the presence or absence of IFNβ.

Incubation 0 h 24 h 48 h 72 h
Cells Control IFNβ Control IFNβ Control IFNβ Control IFNβ
HONE-1 TRAIL-R1 0.02 ± 2.1 0.02 ± 1.0 0.04 ± 2.1 0.07 ± 2.8 0.3 ± 0.4 6.1 ± 0.2 5.8 ± 0.01 15.0 ± 0.2
TRAIL-R2 30.3 ± 3.2 29.1 ± 2.2 29.0 ± 3.1 55.9 ± 3.0 33.0 ± 2.5 72.2 ± 3.3 35.3 ± 4.5 80.3 ± 3.2
HONE-1 EBV TRAIL-R1 0.03 ± 3.4 0.04 ± 1.9 0.08 ± 2.0 0.05 ± 1.3 0.2 ± 0.3 5.5 ± 0.2 6.6 ± 0.01 13.0 ± 0.5
TRAIL-R2 28.8 ± 2.9 27.1 ± 1.9 30.7 ± 4.2 48.0 ± 1.9 31.1 ± 1.2 78.0 ± 2.8 29.0 ± 3.2 89.9 ± 2.2
HK1 TRAIL-R1 0.02 ± 2.7 0.03 ± 3.0 0.1 ± 0.1 0.9 ± 0.3 1.1 ± 0.5 12.3 ± 0.4 12.9 ± 0.2 25.0 ± 0.3
TRAIL-R2 29.9 ± 2.2 32.4 ± 3.0 30.9 ± 2.7 58.0 ± 2.3 35.6 ± 3.1 80.1 ± 3.3 37.7 ± 2.3 88.1 ± 4.1
TW1 TRAIL-R1 0.01 ± 1.3 0.01 ± 2.3 0.1 ± 0.2 0.7 ± 0.2 0.9 ± 0.2 7.0 ± 0.2 7.4 ± 0.2 10.0 ± 0.2
TRAIL-R2 16.0 ± 2.0 16.3 ± 2.9 16.0 ± 3.2 39.3 ± 5.2 18.0 ± 2.5 69.1 ± 2.1 15.8 ± 1.5 84.4 ± 1.4
CNE-2 TRAIL-R1 0.04 ± 2.2 0.1 ± 3.4 0.1 ± 2.3 0.09 ± 1.8 1.1 ± 0.2 12.0 ± 0.7 13.5 ± 0.1 20.0 ± 0.2
TRAIL-R2 40.1 ± 3.1 38.4 ± 2.7 47.6 ± 2.3 46.5 ± 3.0 67.2 ± 5.3 68.8 ± 2.3 77.7 ± 3.3 80.8 ± 3.5
C666-1 TRAIL-R1 0.04 ± 3.3 0.05 ± 2.8 0.06 ± 3.8 0.1 ± 3.5 2.0 ± 0.2 5.2 ± 0.1 5.5 ± 0.2 13.0 ± 0.1
TRAIL-R2 3.0 ± 2.2 4.1 ± 3.4 4.3 ± 4.0 4.5 ± 3.2 3.9 ± 2.2 6.5 ± 2.5 3.9 ± 4.2 6.2 ± 2.5
C17-PDX TRAIL-R1 0.07 ± 3.4 0.09 ± 2.9 2.2 ± 2.0 10.1 ± 2.2 3.0 ± 0.3 18.1 ± 0.1 13.2 ± 0.2 23 ± 0.5
TRAIL-R2 6.0 ± 1.9 5.8 ± 2.2 5.9 ± 2.2 37.7 ± 2.7 6.3 ± 2.5 40.2 ± 3.5 5.3 ± 3.3 60 ± 2.5
NP69 TRAIL-R1 29 ± 0.5 32 ± 0.3 28 ± 0.2 28 ± 0.5 31 ± 0.5 31 ± 0.2 29 ± 0.5 30 ± 0.5
TRAIL-R2 0.05 ± 2.0 0.06 ± 0.9 0.08 ± 1.0 0.04 ± 3.4 4.8 ± 3.5 5.5 ± 3.5 5 ± 2.2 5 ± 2.5

Values are net fluorescent intensities between mean fluorescent intensity of anti-TRAIL-R1 or -R2 antibody and mean fluorescent intensity of respective isotype. Cells were incubated for indicated time periods with or without 1,000 U/ml IFNβ.