Table 1. Surface Expression of TRAIL-R1 and -R2 in NPC cells in the presence or absence of IFNβ.
Incubation | 0 h | 24 h | 48 h | 72 h | |||||
---|---|---|---|---|---|---|---|---|---|
Cells | Control | IFNβ | Control | IFNβ | Control | IFNβ | Control | IFNβ | |
HONE-1 | TRAIL-R1 | 0.02 ± 2.1 | 0.02 ± 1.0 | 0.04 ± 2.1 | 0.07 ± 2.8 | 0.3 ± 0.4 | 6.1 ± 0.2 | 5.8 ± 0.01 | 15.0 ± 0.2 |
TRAIL-R2 | 30.3 ± 3.2 | 29.1 ± 2.2 | 29.0 ± 3.1 | 55.9 ± 3.0 | 33.0 ± 2.5 | 72.2 ± 3.3 | 35.3 ± 4.5 | 80.3 ± 3.2 | |
HONE-1 EBV | TRAIL-R1 | 0.03 ± 3.4 | 0.04 ± 1.9 | 0.08 ± 2.0 | 0.05 ± 1.3 | 0.2 ± 0.3 | 5.5 ± 0.2 | 6.6 ± 0.01 | 13.0 ± 0.5 |
TRAIL-R2 | 28.8 ± 2.9 | 27.1 ± 1.9 | 30.7 ± 4.2 | 48.0 ± 1.9 | 31.1 ± 1.2 | 78.0 ± 2.8 | 29.0 ± 3.2 | 89.9 ± 2.2 | |
HK1 | TRAIL-R1 | 0.02 ± 2.7 | 0.03 ± 3.0 | 0.1 ± 0.1 | 0.9 ± 0.3 | 1.1 ± 0.5 | 12.3 ± 0.4 | 12.9 ± 0.2 | 25.0 ± 0.3 |
TRAIL-R2 | 29.9 ± 2.2 | 32.4 ± 3.0 | 30.9 ± 2.7 | 58.0 ± 2.3 | 35.6 ± 3.1 | 80.1 ± 3.3 | 37.7 ± 2.3 | 88.1 ± 4.1 | |
TW1 | TRAIL-R1 | 0.01 ± 1.3 | 0.01 ± 2.3 | 0.1 ± 0.2 | 0.7 ± 0.2 | 0.9 ± 0.2 | 7.0 ± 0.2 | 7.4 ± 0.2 | 10.0 ± 0.2 |
TRAIL-R2 | 16.0 ± 2.0 | 16.3 ± 2.9 | 16.0 ± 3.2 | 39.3 ± 5.2 | 18.0 ± 2.5 | 69.1 ± 2.1 | 15.8 ± 1.5 | 84.4 ± 1.4 | |
CNE-2 | TRAIL-R1 | 0.04 ± 2.2 | 0.1 ± 3.4 | 0.1 ± 2.3 | 0.09 ± 1.8 | 1.1 ± 0.2 | 12.0 ± 0.7 | 13.5 ± 0.1 | 20.0 ± 0.2 |
TRAIL-R2 | 40.1 ± 3.1 | 38.4 ± 2.7 | 47.6 ± 2.3 | 46.5 ± 3.0 | 67.2 ± 5.3 | 68.8 ± 2.3 | 77.7 ± 3.3 | 80.8 ± 3.5 | |
C666-1 | TRAIL-R1 | 0.04 ± 3.3 | 0.05 ± 2.8 | 0.06 ± 3.8 | 0.1 ± 3.5 | 2.0 ± 0.2 | 5.2 ± 0.1 | 5.5 ± 0.2 | 13.0 ± 0.1 |
TRAIL-R2 | 3.0 ± 2.2 | 4.1 ± 3.4 | 4.3 ± 4.0 | 4.5 ± 3.2 | 3.9 ± 2.2 | 6.5 ± 2.5 | 3.9 ± 4.2 | 6.2 ± 2.5 | |
C17-PDX | TRAIL-R1 | 0.07 ± 3.4 | 0.09 ± 2.9 | 2.2 ± 2.0 | 10.1 ± 2.2 | 3.0 ± 0.3 | 18.1 ± 0.1 | 13.2 ± 0.2 | 23 ± 0.5 |
TRAIL-R2 | 6.0 ± 1.9 | 5.8 ± 2.2 | 5.9 ± 2.2 | 37.7 ± 2.7 | 6.3 ± 2.5 | 40.2 ± 3.5 | 5.3 ± 3.3 | 60 ± 2.5 | |
NP69 | TRAIL-R1 | 29 ± 0.5 | 32 ± 0.3 | 28 ± 0.2 | 28 ± 0.5 | 31 ± 0.5 | 31 ± 0.2 | 29 ± 0.5 | 30 ± 0.5 |
TRAIL-R2 | 0.05 ± 2.0 | 0.06 ± 0.9 | 0.08 ± 1.0 | 0.04 ± 3.4 | 4.8 ± 3.5 | 5.5 ± 3.5 | 5 ± 2.2 | 5 ± 2.5 |
Values are net fluorescent intensities between mean fluorescent intensity of anti-TRAIL-R1 or -R2 antibody and mean fluorescent intensity of respective isotype. Cells were incubated for indicated time periods with or without 1,000 U/ml IFNβ.