Skip to main content
. 2018 Feb 16;9(18):14311–14323. doi: 10.18632/oncotarget.24510

Figure 3. Characterization of 1C11 sub-clones.

Figure 3

(A) Supernatants of 12 sub-clones derived from parental clone 1C11 were incubated with 2 different cell lysates to check for Shh epitope recognition by Western blotting: 1) 293T (endogenous Shh-vector control) and 2) 293T (exogenously transfected with pCMV-Shh) (B) Flow cytometric evaluation of Shh recognition performed with purified antibodies Ab 1C11-2G4, Ab 1C11-2D9 and a commercial (Com) C-term antibody on vector control (pCMV) A549 cells (top panel) and A549 cells transfected with pCMV-Shh (bottom panel) compared with their respective negative controls of cells incubated with secondary antibody alone (Ctrl.). (C) Two sub-clones of 1C11, 2G4 and 2D9, exhibit IgG1, kappa measured using an isotyping kit. Binding affinities (Kd) of antibodies Ab 1C11-2D9 (D) and Ab 1C11-2G4 (E) for recombinant Shh protein.