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. 2018 Feb 16;9(18):14324–14337. doi: 10.18632/oncotarget.24511

Figure 1. HMGA1 expression in intact large (LAE) and small airway epithelium (SAE), basal cells (BC) derived from LAE and SAE, and BC differentiated on air-liquid interface (ALI) culture.

Figure 1

(A) HMGA1 expression in LAE and SAE BC and intact LAE and SAE. Human LAE (3rd-4th order bronchi) of healthy nonsmokers and SAE (10th-12th order) of healthy nonsmokers was obtained by fiberoptic bronchoscopy by brushing. Intact epithelium was directly processed for RNA sequencing (LAE n = 10, SAE n = 22). BC were purified (LAE BC n = 20, SAE BC n = 3) as described in Methods (BC-1). (B) HMGA1 expression over time in ALI culture. Human LAE BC from Lonza were cultured in ALI (see Methods, BC-1 and ALI-1), and RNA harvested at ALI day 0-28. HMGA1 expression (TaqMan) was normalized to 18S rRNA. n = 4 each time point. (C) HMGA1 expression in a normal human LAE assessed by immunohistochemistry (left – HMGA1, right – control IgG).