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. 2018 Feb 21;9(18):14366–14381. doi: 10.18632/oncotarget.24544

Figure 1. TAK1 constitutively associates with TAB1, TAB2 and TAB3 in AGS cells.

Figure 1

Immunoblots of TAK1 (A), TAB2 (B), TAB3 (C) immunoprecipitates, subcellular fractions (D) and cellular lysates (E) are represented. The cells were exposed to H. pylori, TNF or IL-1β for the indicated times (in min). H. pylori lysate (Hp) was used to demonstrate the absence of antibody cross-reactivity with bacterial proteins. In some lysates of the 30 min-infected cells, the immunoprecipitation antibody was not added to approve non-specific protein binding to the beads. The arrows point specific protein bands. (E) Transfection with TAK1 siRNA was performed 48 h prior to stimulation. C, cytosol, CL, cell lysate; Cskn, cytoskeleton; IP, immunoprecipitation; M, membranes; N, nuclei.