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. 2018 Feb 21;9(18):14366–14381. doi: 10.18632/oncotarget.24544

Figure 2. TAK1/TABs complex is required for NF-κB activation in H. pylori- and IL-1β-stimulated cells.

Figure 2

AGS cells were transfected with siRNAs against TAK1 (A) and TAB1 (B, D) or with TAB2 CRISPR/Cas9 and HDR plasmids (C, E). The cells were exposed to H. pylori, TNF or IL-1β for the indicated times (in min). (A–C) Cell lysates were prepared and analyzed via immunoblotting. Densitometry analysis of represented immunoblots was performed. The band intensities (in %) in TAB1 knockdown and TAB2 knockout cells relative to the respective mock values within the infection time course are indicated. (D, E) Cells were transfected with luciferase reporters, treated with H. pylori or cytokines and collected with Passive Lysis buffer for the Transactivation assay. **p < 0.01 vs non-stimulated cells, #p < 0.05 and ##p < 0.01 vs stimulated mock cells.