Figure 5. TRIM28 and CDC37 are involved in regulation of NF-κB and MAP kinases in H. pylori-infected cells.

(A) Mock- or siRNA-transfected AGS cells were infected with H. pylori for the indicated times (in min), and cell lysates were prepared and analyzed by immunoblotting. (B) Densitometry analysis of immunoblots from 3 independent experiments was performed. Graphs represent band intensities (in %) in TRIM28 and CDC37 siRNA-transfected cells relative to the respective mock values within the infection time course. (C) TRIM28 expressing or TRIM28 knockout AGS cells were infected with H. pylori for the indicated times (in min), cell lysates were prepared and analyzed by immunoblotting. ^*p < 0.05 and ^**p < 0.01 vs stimulated mock cells.