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. 2018 Jan 12;9(18):14397–14412. doi: 10.18632/oncotarget.24208

Figure 5. RhoA inhibition decreases AGEs-induced migration in HPMCs.

Figure 5

(A) Morphological changes in HPMCs. After 48h incubation with AGEs, the typical cobblestone-like growth pattern disappears and cells became thin and slender. Fasudil and Y-27632 prevented these changes. (B) Coordinated sheet migration in cells treated with AGEs in the presence or absence of fasudil and Y-27632 (wound-healing assay). B1) Quantitative analysis of wound closure. (C) Transwell migration assay results in HPMCs treated with AGEs with or without pre-treatment with Rho-kinase inhibitors or si-RhoA. Non-treated HPMCs served as controls. C1) Quantitative analysis migration in the transwell assay. (Data are mean ± SD, *P < 0.05 vs control, #P < 0.05 vs AGEs, n = 3).