Figure 3. Calcitriol is able to disrupt the CSC properties.

(a) Isolation of CD44⁻CD117⁻and CD44⁺CD117⁺ cells. CD44⁻CD117⁻and CD44⁺CD117⁺ cells were sorted from 2008 cells using FACS. (b, c) Calcitriol treatment reduces the expression of stem cell specific genes in ovarian CSCs. CD44⁻CD117⁻ (b) and CD44⁺CD117⁺ (c) cells were treated with 5 nM calcitriol for 5 days, mRNA levels of Oct4, Nanog, and Sox2 were determined using qRT-PCR. N = 3, Bar: SD, ^**: P < 0.01.