. 2018 Mar 23;13(3):e0194412. doi: 10.1371/journal.pone.0194412

Table 2. Comparison of RT-qPCR conditions using the pre-existing in-house RT-qPCR assay^*.

Factor	Mastermix^#	Sample volume	Reaction volume	Annealing temperature	Primer concentration	Probe concentration
I Mastermix	1. Superscript-III 2. Express 3. Fastvirus	5μl	1. 25μl 2. 20μl 3. 25μl	60°C	400nM	160nM
II Reaction volume	Superscript-III	1. 5μl 2. 10μl	1. 25μl 2. 50μl	60°C	400nM	160nM
III Sample volume	Superscript-III	1. 10μl 2. 16μl	50μl	60°C	400nM	160nM
III Sample volume	Fastvirus	1. 5μl 2. 15μl	25μl	60°C	400nM	160nM

* All experiments were performed with G1-1326 RNA tenfold dilutions.

^#Superscript-III kit: SuperScript™ III One-Step RT-PCR System with Platinum™ Taq DNA Polymerase; Express kit: EXPRESS One-Step SuperScript® qRT-PCR and Fastvirus kit: TaqMan® Fast Virus 1-Step.

Table 2. Comparison of RT-qPCR conditions using the pre-existing in-house RT-qPCR assay*.

Table 2. Comparison of RT-qPCR conditions using the pre-existing in-house RT-qPCR assay^*.