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Immunohistochemistry assay demonstrating RBP4 expression in the EPM. RBP4 was significantly decreased at E13.5 and E14.5 in the EPM exposed to atRA when compared with the Con group. (A) Immunohistochemistry results (scale bar, 50 µm), (B) quantification. ***P<0.001. RBP4, retinol binding protein 4; EPM, embryonic palatal mesenchyme; E, embryonic day; atRA, all-trans retinoic acid; Con, control.
