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EAS decreases the expression of MMP-3 and MMP-13 in TNF-α-treated chondrocytes. Chondrocytes were treated with or without TNF-α, and with or without EAS, for 48 h, and the expression of (A) MMP-3 and (B) MMP-13 was determined by immunofluorescent staining (green). The nuclei of chondrocytes were stained with DAPI (blue). The results represent four independent experiments. EAS, electroacupuncture serum; MMP, matrix metalloproteinase; TNF-α, tumor necrosis factor-α.
