Skip to main content
. 2017 Aug 23;16(5):5847–5854. doi: 10.3892/mmr.2017.7344

Figure 2.

Figure 2.

Birc2 is a target of miR-205 and downregulation of miR-205 reduces cell apoptosis in H9c2 cells. (A) Sequence alignment of miR-205 and 3′UTR of Birc2. (B) 3′UTR of Birc2 or a mutated sequence was cloned into pmiR-Glo vector. (C) Luciferase reporter assay with co-transfection of wild-type or mutant 3′UTR and miR-205m or NCm in 293T cells. (D) Protein and (E) mRNA expression levels of Birc2, caspase-3, Bax and Bcl-2 in H9c2 cells transfected with miR-205 as an internal control. β-actin served as a loading control. Data are expressed as the mean ± standard deviation. *P<0.05 vs. NCm; #P<0.05 vs. NCi. miR-205m, miR-205 mimics; miR-205i, miR-205 inhibitor; NCm, negative control mimics; NCi, negative control inhibitor; Birc2, baculoviral IAP repeat-containing protein 2; miR, microRNA; 3′UTR, 3-untranslated region; Con, control; WT, wild-type; Bax, Bcl-2-associated X protein; Bcl-2, B-cell lymphoma 2.