Figure 5.

Placental explants were cultured on Matrigel under various culture conditions. (A) The growth of placental explants was captured under a light microscope (a-1, b-1, c-1, d-1). Scale bar, 100 µm. GPR30 expression was detected using immunohistochemical staining in placental explants (a-2, b-2, c-2, d-2). Scale bar, 100 µm. (a-1 and a-2) Control; (b-1 and b-2) H/R; (c-1 and c-2) H/R+E2; (d-1 and d-2) H/R+G1. (B) When compared with normoxic conditions (control), the outgrowth of placental explants was significantly reduced in H/R conditions. Treatment with G1 significantly increased the outgrowth of placental explants in H/R conditions. H/R, hypoxia/reoxygenation; GPR30, G-protein coupled receptor 30; E2, 17β-estrogen.