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. 2017 Aug 22;16(5):6034–6040. doi: 10.3892/mmr.2017.7319

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Copyright: © Qian et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 5. — Notch pathway inhibitor MK-0752 reverses the effects of IRF4 on NSCLC cell growth (A) Notch pathway inhibitor MK-0752 reverses the effect of IRF4 on proliferation of A549 cells. A549 cell were infected with lentivirus expressing Ctrl or IRF4 vectors with/without MK-0752 (20 µM) treatment. Relative cell numbers were evaluated at the indicated time points using MTT method. *P<0.05 and **P<0.01 vs. Ctrl. ^#P<0.05 indicates IRF4+MK-0752 vs. IRF4. (B) Notch pathway inhibitor MK-0752 reverses the effect of IRF4 on proliferation of LC-AI cells. LC-AI cells were infected with lentivirus expressing Ctrl or IRF4 vectors with/without MK-0752 (20 µM) treatment. Relative cell numbers were evaluated at the indicated time points using MTT method. *P<0.05 and **P<0.01 vs. Ctrl. ^#P<0.05 indicates IRF4+MK-0752 vs. IRF4. (C) Notch pathway inhibitor MK-0752 reverses the effect of IRF4 on colony formation of A549 cells. A549 cells were infected with lentivirus expressing Ctrl or IRF4 lentivirus vectors with/without MK-0752 (20 µM) treatment. The colony number in each well was evaluated two weeks later by soft sugar colony formation assay. **P<0.01 vs. Ctrl. ^#P<0.05 indicates IRF4+MK-0752 vs. IRF4. (D) Notch pathway inhibitor MK-0752 reverses the effect of IRF4 on colony formation of LC-AI cells. A549 cells were infected with lentivirus expressing Ctrl or IRF4 lentivirus vectors with/without MK-0752 (20 µM) treatment. The colony number in each well was evaluated two weeks later by soft sugar colony formation assay. **P<0.01 vs. Ctrl. ^#P<0.05 indicates IRF4+MK-0752 vs. IRF4. IRF4, interferon regulatory factor 4; NSCLC, non-small cell lung cancer, sh-Ctrl, negative control; Ctrl, control.