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. 2017 Aug 31;16(5):6526–6531. doi: 10.3892/mmr.2017.7416

Table I.

Primers used for specific truncated WASP gene detection for the proband and a healthy control by polymerase chain reaction amplification.

Primer Sequence (5′-3′) Product length (bp)
WAS-1F TCTAAGCAGTCAAGTGGAGGAG   930
WAS-1R ATCTGGATGAGTCTTTGGTTCTG
WAS-2F GAGCCTCAACTTCCTAAGACTAGA 1,063
WAS-2R TCAGCCATCTACCGCCAATC
WAS-3F TACCTCCATGACCATCCAACA   380
WAS-3R CCATCCTTCCATTCACTCAGC
WAS-4F TTCCATAACTCCTGCCTATACTCA   680
WAS-4R CACTGACCAACTCCTGACTGA
WAS-5AF TCACTCAGTCCTTATGGGAGCACCT 1,021
WAS-5AR TCAAACAGATGGGGCTGATGTCACT
WAS-6F TTAACCAGACAGGAAGCAAT   593
WAS-6R CTTGAGTGAAGAGAACTGAGA

F, forward; R, reverse; WASP, Wiskott-Aldrich syndrome protein.