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Primers used for specific truncated WASP gene detection for the mother and a healthy control by quantitative polymerase chain reaction amplification.
| Primer | Sequence (5′-3′) | Product length (bp) |
|---|---|---|
| WAS-1QF | AAGACCTTGTGGCTACCCCT | 144 |
| WAS-1QR | AGCACACAGCCCCACAATGCTC | |
| WAS-3QF | GTCAATGAGCCAACCACCCTA | 151 |
| WAS-3QR | TTCTTATCAGCTGGGCTAGGTC | |
| WAS-5QF | CTAAGCCCTCTGTGCTGATCCC | 136 |
| WAS-5QR | GGCTCTGCTTCTCTTCTGCATCAC |
F, forward; R, reverse; WASP, Wiskott-Aldrich syndrome protein.
