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. 2017 Sep 5;16(5):6729–6735. doi: 10.3892/mmr.2017.7422

Figure 2.

Figure 2.

VEGFA is a direct target of miR-613. (A) Sequence alignment of miR-613 and the VEGFA 3′-UTR, which contains one predicted miR-613-biding site. Luciferase assay in (B) U87 and (C) U251 cell lines co-transfected with miR-613 and a luciferase reporter containing the VEGFA 3′-UTR WT or a Mut. (D) mRNA and (E) protein expression levels of VEGFA following miR-613 transfection. Data are expressed as the mean ± standard error. *P<0.05. miR, microRNA; WT, wild-type; Mut, mutant; VEGFA, vascular endothelial growth factor A; UTR, untranslated region.