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. 2017 Sep 5;16(5):6729–6735. doi: 10.3892/mmr.2017.7422

Figure 3.

Figure 3.

miR-613 suppresses cell proliferation and angiogenesis through targeting VEGFA. (A) Quantification of colony numbers of U251 and U87 cells following miR-163 or VEGFA transfection. (B) Representative images of colony formation assay using U251 and U87 cells transfected with control miRNA, miR-613 mimics, or miR-613 mimics and VEGFA. (C) Quantification of the invasive ability of U87 and U251 cells. (D) Representative images of invasion assay using U87 and U251 cells. Magnification, ×100. (E) Tube formation of endothelial HUVEC cells after treatment with medium preconditioned by miR-613-overexpressing U87 and U251 cells or mock cells. (F) Representative western blot images of the protein expression levels of VEGFA and Ang-2 in U87 and U251 cells transfected with mimic control, miR-613 mimics or miR-613, and VEGFA. GAPDH served as a reference protein. Data are expressed as the mean ± standard error. *P<0.05. miR, microRNA; VEGFA, vascular endothelial growth factor A; Ang-2, angiopoietin-2.