Table I.
Primers designed using Primer 6.0 software and synthesized from Generay Biotech.
| Gene name | Primer sequence (5′ to 3′) | Amplicon size/NCBI.NM |
|---|---|---|
| H-GAPDH-157 bp-F | GAGTCCACTGGCGTCTTCAC | 157 bp |
| H-GAPDH-157 bp-R | TGCTGATGATCTTGAGGCTGTT | NM_001256799.2 |
| H-CX37-F | AGTTCCTCTTCGTCAGCACAC | 239 bp |
| H-CX37-R | GAGCACACTGGCGACATAGG | NM_002060.2 |
| H-CX40-F | TGGAAGAAGATCAGACAGCGATT | 218 bp |
| H-CX40-R | CCTCGTACTTGCTCGGTGAC | NM_005266.6 |
| H-CX43-F | CTGGTGGTGTCCTTGGTGTC | 182 bp |
| H-CX43-R | GGTGAGGAGCAGCCATTGAA | NM_000165.4 |
| H-CX45-F | ACCGAACTGTCCAATGCTAAGA | 142 bp |
| H-CX45-R | AGCGTTCCTGAGCCATCCT | NM_001080383.1 |
| H-CX46-F | TGTTCATCTTCCGCATCTTGGT | 119 bp |
| H-CX46-R | CCTGTCGTAGCAGACGTTCTC | NM_021954.3 |
| H-Claudin-1-F | AGGTCTTGCCGCCTTGGTA | 173 bp |
| H-Claudin-1-R | GACAGGAACAGGAGAGCAGTG | NM_001307.5 |
| H-Endoglin-F | CGACGCCAACCACAACAT | 156 bp |
| H-Endoglin-R | ACGAAGGATGCCACAATGC | NM_000118.3 |
| H-Occludin-1-F | TCGCTGCCAATGCTCATCTG | 206 bp |
| H-Occludin-1-R | GCCTCCAAGGAAGAGACTGAAG | NM_005985.3 |
| H-SMAD-F | CATGCCACTCAACGCCACTT | 295 bp |
| H-SMAD-R | AACCGCCTGAACATCTCCTCT | NM_005900.2 |
| H-ZO-1-F | GCGGATGGTGCTACAAGTGAT | 138 bp |
| H-ZO-1-R | GCCTTCTGTGTCTGTGTCTTCA | NM_001301025.1 |
GAPDH, glyceraldehyde 3-phosphate dehydrogenase; bp, base pairs; F, forward; R, reverse; SMAD, mothers against decapentaplegic homolog; ZO-1, tight junction protein 1.