. 2017 Sep 13;16(5):6828–6836. doi: 10.3892/mmr.2017.7493

Table I.

Summary statistics and identifications of differentially-expressed plasma metabolites and their metabolic pathways.

						H0 group^b	H1 group^b	H4 group^b		P-value for ANOVA

No.	tr/min	m/z	Adduct	Metabolite	Associated pathway	n=10	n=10	n=10	VIP^c	H1 & H0	H4 & H0	H4 & H1
ESI
(+)
1	1.13	118.086	M+H	Valine^a	Valine, leucine and isoleucine biosynthesis	0.0523±0-.0082	0.0650±0.0108	0.1203±0.0236	1.36	0.026	P<0.01	P<0.01
2	1.16	204.123	M+H	Acetylcarnitine	Fatty acid transportation	0.0965±0.0109	0.0743±0.0106	0.0425±0.0090	1.21	P<0.01	P<0.01	P<0.01
3	1.17	150.058	M+H	Methionine^a	Cysteine and methionine metabolism	0.0401±0.0048	0.0479±0.0100	0.0954±0.0181	1.24	0.117	P<0.01	P<0.01
4	1.63	182.081	M+H	Tyrosine	Tyrosine metabolism	0.0448±0.0080	0.0748±0.0139	0.1208±0.0292	1.39	P<0.01	P<0.01	0.002
5	1.80	132.102	M+H	Isoleucine	Valine, leucine and isoleucine biosynthesis	0.2824±0.0432	0.4661±0.1708	0.6779±0.1256	3.09	0.023	P<0.01	0.017
6	3.64	166.086	M+H	Phenylalanine^a	Phenylalanine metabolism	0.1812±0.0179	0.2628±0.0472	0.3334±0.0413	2.01	0.001	P<0.01	0.007
7	14.62	494.325	M+H	LysoPC(16:1)	Glycerophospholipid metabolism	0.0488±0.0085	0.1010±0.0244	0.0672±0.0179	1.09	P<0.01	0.032	0.008
8	15.16	520.341	M+H	LysoPC(18:2)	Glycerophospholipid metabolism	1.4183±0.2117	2.2970±0.3396	1.5134±0.2553	4.84	P<0.01	1.000	P<0.01
9	15.20	568.341	M+H	LysoPC(22:6)	Glycerophospholipid metabolism	0.2987±0.0414	0.4279±0.1073	0.2297±0.0420	2.23	0.012	0.005	P<0.01
10	15.22	544.341	M+H	LysoPC(20:4)	Glycerophospholipid metabolism	0.4145±0.0396	0.7173±0.2747	0.4391±0.1241	2.47	0.019	0.907	0.035
11	15.44	424.343	M+H	Linoelaidylcarnitine	Fatty acid transportation	0.0242±0.0061	0.0633±0.0257	0.0529±0.0161	1.10	0.002	0.001	0.633
12	15.77	546.356	M+H	LysoPC(20:3)	Glycerophospholipid metabolism	0.0910±0.0171	0.2249±0.0473	0.1192±0.0485	1.84	P<0.01	0.281	P<0.01
13	15.91	400.343	M+H	Palmitoylcarnitine	Fatty acid transportation	0.0632±0.0113	0.0864±0.0211	0.1100±0.0151	1.05	0.011	P<0.01	0.010
14	16.14	522.357	M+H	LysoPC(18:1)	Glycerophospholipid metabolism	0.8837±0.1314	1.4495±0.3031	1.0217±0.2578	3.50	P<0.01	0.380	0.010
15	16.24	426.359	M+H	Octadecenoylcarnitine	Fatty acid transportation	0.0635±0.0086	0.1158±0.0222	0.1484±0.0194	1.48	P<0.01	P<0.01	0.008
ESI
(−)
1	0.74	124.008	M-H	Taurine	Taurine and hypotaurine metabolism	0.3305±0.0689	0.3886±0.0563	0.6527±0.0923	1.10	0.272	P<0.01	P<0.01
2	0.82	133.015	M-H	Malate	Citrate cycle (TCA cycle)	0.2442±0.1123	0.4746±0.0960	0.1731±0.0642	1.53	P<0.01	0.297	P<0.01
3	1.20	167.021	M-H	Uric acid	Purine metabolism	0.4654±0.0871	1.2867±0.1555	0.6374±0.1670	2.72	P<0.01	0.033	P<0.01
4	1.28	191.020	M-H	Citrate^a	Citrate cycle (TCA cycle)	0.7793±0.2188	1.7729±0.2274	1.1371±0.2382	1.83	P<0.01`	0.005	P<0.01
5	1.58	117.019	M-H	Succinate^a	Citrate cycle (TCA cycle)	0.1866±0.1333	1.1098±0.2954	0.1141±0.0424	3.16	P<0.01	0.324	P<0.01
6	16.51	319.229	M-H	15-HETE	Arachidonic acid metabolism	2.1342±0.6468	3.0665±0.5586	2.0695±1.0005	2.40	0.009	0.997	0.044
7	19.33	303.233	M-H	Arachidonic acid^a	Arachidonic acid metabolism	0.5118±0.0825	0.7511±0.0553	0.7187±0.1332	1.41	P<0.01	P<0.01	1.000
8	19.50	279.233	M-H	Linoleic acid^a	Linoleic acid metabolism	0.3468±0.0589	0.8998±0.2997	0.5230±0.1105	1.46	0.001	0.002	0.009
9	20.23	255.233	M-H	Palmitic acid^a	Fatty acid metabolism	0.1555±0.0547	0.4895±0.1369	0.2829±0.0701	1.12	P<0.01	0.016	P<0.01
10	20.47	281.249	M-H	Oleic acid^a	Fatty acid metabolism	0.2162±0.0927	0.9256±0.3168	0.4428±0.1319	1.70	P<0.01	0.001	0.002

Metabolites validated by reference standards.

Data are presented as the mean ± standard deviation.

VIP was obtained from partial least squares-discriminant analysis with a threshold of 1.0. 15-HETE, 15-hydroxyeicosatetraenoic acid; ANOVA, analysis of variance; TCA, tricarboxylic acid; VIP, variable importance in the projection; ESI+, positive ion mode; ESI-, negative ion mode.